Detaljerad analys av proteintopologi av extracellulära vesiklar

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The 567 proteins up-regulated in 3D Additionally, these proteins are registered in Vesiclepedia [28,29] in the list of the top 100 proteins previously found in EVs. In total, of the EV GABARAP proxitome, more than 95% is registered in Vesiclepedia (Supplementary Figure S9A), and 63 proteins were found in the Vesiclepedia top 100 (Supplementary Figure S9B). 5. Supplementary table S1B has been edited and now lists the top 100 EV proteins listed in Vesiclepedia. Exocarta has been replaced in the manuscript text with Vesiclepedia (line 131) 6. We have added the following: “The serum-EV cohorts used in this study are detailed in Table S2A. The methods for serum processing, EV isolation, EV Top Ingenuity Pathway Analysis (IPA ) interactome derived from transcriptional regulators found in the Vesiclepedia database. Proteins clustered within the Top Networks/Associated Functions as derived from IPA algorithms are shown as members of this interactome, which was entitled “Gene Expression, Cell Cycle, Infectious Disease.” Download top 100 proteins identified in extracellular vesicles: 14: Completed: 13: Allow users to control the databases: 14: Completed: 14: Download all proteins/RNA identified in a subtype of extracellular vesicle: 13: Completed: 15: Enrichment of genes in different cancer types using COSMIC data: 13: Completed: 16: Save workspace or session When we compared the EV proteins with the top 100 marker proteins from ExoCarta and Vesiclepedia, we found that of the 100 markers provided in the two databases, more than 74 proteins were observed in the EVs from 2D and 3D cultured hMSCs, those included CD9, CD63, CD81, Syntenin-1, and Flotillin-1 (Fig.

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November 2018; database pr ovides top 100 pr oteins that are often identied. When we compared the EV proteins with the top 100 marker proteins from ExoCarta and Vesiclepedia, we found that of the 100 markers provided in the two databases, more than 74 proteins were observed in the EVs from 2D and 3D cultured hMSCs, those included CD9, CD63, CD81, Syntenin-1, and Flotillin-1 (Fig. 5G).

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Här undersöker vi topologin för proteinlasten som finns i EVs, eftersom denna är välorganiserade i EV-databaserna EVpedia 14, 15 och Vesiclepedia 16 . 100 enheter / ml Penicillin, 100 ug / ml Streptomycin (HyClone) och 1, 2 U / ml  01/09/2013 - Vesiclepedia Version 2.1 released!! 01/09/2013 - Addition of new datasets to Vesiclepedia. 01/04/2013 - Vesiclepedia highlighted in Nature Methods.

Proteins clustered within the Top Networks/Associated Functions as derived from IPA algorithms are shown as members of this interactome, which was entitled “Gene Expression, Cell Cycle, Infectious Disease.” Download top 100 proteins identified in extracellular vesicles: 14: Completed: 13: Allow users to control the databases: 14: Completed: 14: Download all proteins/RNA identified in a subtype of extracellular vesicle: 13: Completed: 15: Enrichment of genes in different cancer types using COSMIC data: 13: Completed: 16: Save workspace or session When we compared the EV proteins with the top 100 marker proteins from ExoCarta and Vesiclepedia, we found that of the 100 markers provided in the two databases, more than 74 proteins were observed in the EVs from 2D and 3D cultured hMSCs, those included CD9, CD63, CD81, Syntenin-1, and Flotillin-1 (Fig. 5G).
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01/09/2013 - Addition of new datasets to Vesiclepedia. 01/04/2013 - Vesiclepedia highlighted in Nature Methods. 01/04/2013 - Manuscript describing Vesiclepedia was highlighted in the tool section of Nature Methods. 19/12/2012 - Vesiclepedia published in PLOS Biology The data is free to all. Please cite the article describing Vesiclepedia if you use the data. Vesiclepedia citation: 1, Pathan, M., Fonseka, P., Chitti, S.V., Kang, T However, we emphasise caution when the data from Vesiclepedia is used for EV based analysis.

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